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bmp10 elisa kit  (R&D Systems)


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    R&D Systems bmp10 elisa kit
    Bmp10 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmp10+duoset+elisa/Human%2FMouse+BMP-10+DuoSet+ELISA/pm41222740-105-11-17
    Average 93 stars, based on 5 article reviews
    bmp10 elisa kit - by Bioz Stars, 2026-09
    93/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Combined Effects of Bone Morphogenetic Protein 10 and Crossveinless-2 on Cardiomyocyte Differentiation in Mouse Adipocyte-Derived Stem Cells
    Article Snippet: .. There was no detectable BMP10 in the culture medium (20% FBS) when tested by BMP10 DuoSet ELISA (R&D Systems, mature bovine BMP10 is 100% identical to human BMP10). ..



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    Figure 1. Plasma BMP9, <t>BMP10,</t> and BMP9/10 concentrations do not differ between pulmonary inflow and systemic venous circulation. Plasma from controls was sampled from the right atrium (RA), pulmonary artery (PA), aorta, SVC, and infrahepatic IVC and ligands quantified by sandwich ELISA. All values are GFD equivalents. Colored lines connect samples from a single patient. Not significant by repeated measures one- way ANOVA: BMP9, N = 31, P=0.13; BMP10, N=31, P=0.17; BMP9/10, N=21, P=0.21.
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    Mouse DFAT cells were treated with growth medium supplemented with control vehicle, <t>BMP10</t> (25 ng/ml), CV2 (50 ng/ml), or BMP10 for 3 days followed by CV2 for 3 days (BMP10/CV2). Photos were obtained after 5, 7, 10 and 14 days.
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    Image Search Results


    Figure 1. Plasma BMP9, BMP10, and BMP9/10 concentrations do not differ between pulmonary inflow and systemic venous circulation. Plasma from controls was sampled from the right atrium (RA), pulmonary artery (PA), aorta, SVC, and infrahepatic IVC and ligands quantified by sandwich ELISA. All values are GFD equivalents. Colored lines connect samples from a single patient. Not significant by repeated measures one- way ANOVA: BMP9, N = 31, P=0.13; BMP10, N=31, P=0.17; BMP9/10, N=21, P=0.21.

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: In Search of “Hepatic Factor”: Lack of Evidence for ALK1 Ligands BMP9 and BMP10

    doi: 10.1164/rccm.202005-1937le

    Figure Lengend Snippet: Figure 1. Plasma BMP9, BMP10, and BMP9/10 concentrations do not differ between pulmonary inflow and systemic venous circulation. Plasma from controls was sampled from the right atrium (RA), pulmonary artery (PA), aorta, SVC, and infrahepatic IVC and ligands quantified by sandwich ELISA. All values are GFD equivalents. Colored lines connect samples from a single patient. Not significant by repeated measures one- way ANOVA: BMP9, N = 31, P=0.13; BMP10, N=31, P=0.17; BMP9/10, N=21, P=0.21.

    Article Snippet: Published September 01, 2020 as 10.1164/rccm.202005-1937LE Copyright © 2020 by the American Thoracic Society BMP9/10 GFD (Fig. 1), but not BMP10 GFD (DY2926, R&D Systems; data not shown).

    Techniques: Clinical Proteomics, Sandwich ELISA

    Figure 2. Plasma BMP9, BMP10, and BMP9/10 concentrations are lower in Glenn cases compared to controls. Within-patient data were averaged across all sampling sites (RA, PA, aorta, SVC, IVC) for controls and Glenn cases and evaluated by Welch’s t-test. Error bars, mean SD. BMP9: control, N=31; Glenn, N=5; P=0.02. BMP10: control, N=31; Glenn, N=7; P=0.0006. BMP9/10: control, N=21; Glenn, N=6; P=0.01. Open circles indicate cases with PAVMs.

    Journal: American Journal of Respiratory and Critical Care Medicine

    Article Title: In Search of “Hepatic Factor”: Lack of Evidence for ALK1 Ligands BMP9 and BMP10

    doi: 10.1164/rccm.202005-1937le

    Figure Lengend Snippet: Figure 2. Plasma BMP9, BMP10, and BMP9/10 concentrations are lower in Glenn cases compared to controls. Within-patient data were averaged across all sampling sites (RA, PA, aorta, SVC, IVC) for controls and Glenn cases and evaluated by Welch’s t-test. Error bars, mean SD. BMP9: control, N=31; Glenn, N=5; P=0.02. BMP10: control, N=31; Glenn, N=7; P=0.0006. BMP9/10: control, N=21; Glenn, N=6; P=0.01. Open circles indicate cases with PAVMs.

    Article Snippet: Published September 01, 2020 as 10.1164/rccm.202005-1937LE Copyright © 2020 by the American Thoracic Society BMP9/10 GFD (Fig. 1), but not BMP10 GFD (DY2926, R&D Systems; data not shown).

    Techniques: Clinical Proteomics, Sampling, Control

    Mouse DFAT cells were treated with growth medium supplemented with control vehicle, BMP10 (25 ng/ml), CV2 (50 ng/ml), or BMP10 for 3 days followed by CV2 for 3 days (BMP10/CV2). Photos were obtained after 5, 7, 10 and 14 days.

    Journal: Journal of cellular physiology

    Article Title: Combined Effects of Bone Morphogenetic Protein 10 and Crossveinless-2 on Cardiomyocyte Differentiation in Mouse Adipocyte-Derived Stem Cells

    doi: 10.1002/jcp.25983

    Figure Lengend Snippet: Mouse DFAT cells were treated with growth medium supplemented with control vehicle, BMP10 (25 ng/ml), CV2 (50 ng/ml), or BMP10 for 3 days followed by CV2 for 3 days (BMP10/CV2). Photos were obtained after 5, 7, 10 and 14 days.

    Article Snippet: There was no detectable BMP10 in the culture medium (20% FBS) when tested by BMP10 DuoSet ELISA (R&D Systems, mature bovine BMP10 is 100% identical to human BMP10).

    Techniques: Control

    Mouse DFAT cells were treated with growth medium supplemented with control vehicle (day 5-7), BMP10 (25 ng/ml, day 5-7), CV2/5-7 (50 ng/ml, day 5-7), BMP10 (day 5-7) followed by CV2 (day 8-10) (BMP10/CV2), anti-BMP10 antibodies (100 ng/ml, day 5-7), or CV2/8-10 (50 ng/ml, day 8-10).

    Journal: Journal of cellular physiology

    Article Title: Combined Effects of Bone Morphogenetic Protein 10 and Crossveinless-2 on Cardiomyocyte Differentiation in Mouse Adipocyte-Derived Stem Cells

    doi: 10.1002/jcp.25983

    Figure Lengend Snippet: Mouse DFAT cells were treated with growth medium supplemented with control vehicle (day 5-7), BMP10 (25 ng/ml, day 5-7), CV2/5-7 (50 ng/ml, day 5-7), BMP10 (day 5-7) followed by CV2 (day 8-10) (BMP10/CV2), anti-BMP10 antibodies (100 ng/ml, day 5-7), or CV2/8-10 (50 ng/ml, day 8-10).

    Article Snippet: There was no detectable BMP10 in the culture medium (20% FBS) when tested by BMP10 DuoSet ELISA (R&D Systems, mature bovine BMP10 is 100% identical to human BMP10).

    Techniques: Control

    Mouse DFAT cells were treated with growth medium supplemented with control vehicle (day 5-7), BMP10 (25 ng/ml, day 5-7), CV2/5-7 (50 ng/ml, day 5-7), BMP10 (day 5-7) followed by CV2 (day 8-10) (BMP10/CV2). Cell morphology and expression of sarcomeric alpha-actinin (Sr-alpha-actinin, green) and Troponin I (Tr-I, red) were examined after 14 days by immunofluorescence. DAPI (blue) was used to visualize nuclei.

    Journal: Journal of cellular physiology

    Article Title: Combined Effects of Bone Morphogenetic Protein 10 and Crossveinless-2 on Cardiomyocyte Differentiation in Mouse Adipocyte-Derived Stem Cells

    doi: 10.1002/jcp.25983

    Figure Lengend Snippet: Mouse DFAT cells were treated with growth medium supplemented with control vehicle (day 5-7), BMP10 (25 ng/ml, day 5-7), CV2/5-7 (50 ng/ml, day 5-7), BMP10 (day 5-7) followed by CV2 (day 8-10) (BMP10/CV2). Cell morphology and expression of sarcomeric alpha-actinin (Sr-alpha-actinin, green) and Troponin I (Tr-I, red) were examined after 14 days by immunofluorescence. DAPI (blue) was used to visualize nuclei.

    Article Snippet: There was no detectable BMP10 in the culture medium (20% FBS) when tested by BMP10 DuoSet ELISA (R&D Systems, mature bovine BMP10 is 100% identical to human BMP10).

    Techniques: Control, Expressing, Immunofluorescence

    Mouse DFAT cells were treated with growth medium supplemented with control vehicle (day 5-7), BMP10 (25 ng/ml, day 5-7), CV2/5-7 (50 ng/ml, day 5-7), BMP10 (day 5-7) followed by CV2 (day 8-10) (BMP10/CV2). Cell morphology and expression of sarcomeric alpha-actinin (Sr-alpha-actinin, green) and Troponin I (Tr-I, red) were examined after 3 weeks by immunofluorescence. DAPI (blue) was used to visualize nuclei.

    Journal: Journal of cellular physiology

    Article Title: Combined Effects of Bone Morphogenetic Protein 10 and Crossveinless-2 on Cardiomyocyte Differentiation in Mouse Adipocyte-Derived Stem Cells

    doi: 10.1002/jcp.25983

    Figure Lengend Snippet: Mouse DFAT cells were treated with growth medium supplemented with control vehicle (day 5-7), BMP10 (25 ng/ml, day 5-7), CV2/5-7 (50 ng/ml, day 5-7), BMP10 (day 5-7) followed by CV2 (day 8-10) (BMP10/CV2). Cell morphology and expression of sarcomeric alpha-actinin (Sr-alpha-actinin, green) and Troponin I (Tr-I, red) were examined after 3 weeks by immunofluorescence. DAPI (blue) was used to visualize nuclei.

    Article Snippet: There was no detectable BMP10 in the culture medium (20% FBS) when tested by BMP10 DuoSet ELISA (R&D Systems, mature bovine BMP10 is 100% identical to human BMP10).

    Techniques: Control, Expressing, Immunofluorescence

    (A, B) Mouse DFAT cells were plated in 6-well culture dishes and treated with growth medium supplemented with control vehicle, BMP10 (25 ng/ml), CV2 (50 ng/ml), or BMP10 for 3 days followed by CV2 for 3 days (BMP10/CV2). After 3 weeks, the cells were immunostained for Troponin I. For each treatment, all stained cells and colonies in one entire 6-well were counted by fluorescence microscopy. (A) The cells were classified as small round cells (<50 μm), or short (50-200 μm), medium (200-500 μm), or long myotubes (>500 μm), and (B) colonies were classified as small (1-25 myotubes), medium (25-50 myotubes) or large (>50 myotubes) colonies (representative of 3 experiments).

    Journal: Journal of cellular physiology

    Article Title: Combined Effects of Bone Morphogenetic Protein 10 and Crossveinless-2 on Cardiomyocyte Differentiation in Mouse Adipocyte-Derived Stem Cells

    doi: 10.1002/jcp.25983

    Figure Lengend Snippet: (A, B) Mouse DFAT cells were plated in 6-well culture dishes and treated with growth medium supplemented with control vehicle, BMP10 (25 ng/ml), CV2 (50 ng/ml), or BMP10 for 3 days followed by CV2 for 3 days (BMP10/CV2). After 3 weeks, the cells were immunostained for Troponin I. For each treatment, all stained cells and colonies in one entire 6-well were counted by fluorescence microscopy. (A) The cells were classified as small round cells (<50 μm), or short (50-200 μm), medium (200-500 μm), or long myotubes (>500 μm), and (B) colonies were classified as small (1-25 myotubes), medium (25-50 myotubes) or large (>50 myotubes) colonies (representative of 3 experiments).

    Article Snippet: There was no detectable BMP10 in the culture medium (20% FBS) when tested by BMP10 DuoSet ELISA (R&D Systems, mature bovine BMP10 is 100% identical to human BMP10).

    Techniques: Control, Staining, Fluorescence, Microscopy